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KMID : 0644120100230010001
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Journal of Oriental Gynecology
2010 Volume.23 No. 1 p.1 ~ p.11
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Experimental Studies on Antimetastatic and Immunomodulating Effects of Ulmus davidiana
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Kim Heung-Soo
Lee Kyung-Sub
Lee Chang-Hoon
Lee Jin-Moo
Cho Jung-Hoon
Jang Jun-Bock
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Abstract
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Purpose: This study was designed to investigate the antimetastatic and immunomodulating effects of extracts of Ulmus davidiana extracts(U. D. Ex.).
Methods: Antimetastatic experiments were conducted in vitro and in vivo by using colon 26-M3.1 carcinoma, L5178Y-R lymphoma cell and Hela cell. To observe the immunomodulating effects of U. D. Ex., we measured IL-6, IL-10, IL-12 and TNF-α from peritoneal macrophages. And we evaluated the activation of NK cell by using anti-asialo-GM1 serum.
Results: We found that the administration of U. D. Ex. significantly inhibited tumor metastasis in vivo. In vitro cytotoxicity analysis, cell growth are closer to 100% in case of Colon 26-M3.1 carcinoma, L5178Y-R lymphoma cell and Hela cell at low concentration. In case of macrophage, cell proliferation is closer to 100% less than 250㎍/㎖ of U. D. Ex.. The level of cytokine such as IL-6, IL-10, IL-12 which stimulates U. D. Ex. was increased in dose-dependent manner compared to the control group. In case of TNF-α, the level was increased at concentration of 1,000㎍/㎖. The depletion of NK cells by anti-asialo GM1 serum partly abolished the inhibitory effect of U. D. Ex. on tumor metastasis.
Conclusion: Ulmus davidiana appears to have considerable activity on the anti-metastasis by activation the immune system.
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KEYWORD
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Ulmus davidiana, Anti-metastasis, Immune system
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